10.10. Methods of hydro-biological tests
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10.10. Methods of hydro-biological tests
While testing phyto-plankton, we calculated numbers of water plants in Fuchs-Rosental chamber by filling it three times and then taking a mean value. For trichomes, cenobias and colonies, we calculated numbers of cells. Bio-masses were determined by calculating volumes of water plants according to generally accepted methods.
Quantitative processing of zoo-plankton samples was carried out in Bogorov chamber by total calculation of organisms in several parts of sample, and then by studying the whole sample in order to find single species and measure lengths of zoo-plankters, to calculate individual masses of organisms of all species according to parameters of masses-lengths correspondence. Weight of the коловратки was determined according to literary sources. Bio-masses of zoo-plankton were calculated by multiplying an individual weight of organisms of each kind by their number.
Bio-masses of separate macro-zoo-bentos organisms or groups were determined by weighing them after drying them off on a sheet of filter paper to the condition when there were no wet spots, then we calculated the bio-masses for 1 м 2 bottom area. Quantities and bio-masses of big molluscs were not taken into account while testing quantitative samples.
In order to assess quality of surface waters and bottom sediments (by testing phyto- and zoo-plankton communities), we used the saprobe index by Pantle and Bukke modified by Sladechek and the index of species variety by Shannon. The corresponding bio-identification was carried out according to the generally accepted methods of Woodywiss biotic indices (on taxonomic variety and revealing values of hydrobionts) and to the index by Goodnight-Whitley (on relative number of Oligochaeta).
Water quality classification (on hydro-biological parameters) was carried out in accordance with the standards 17.1.3.07.82.



